AB Vector is a leader in the design and development of novel vectors facilitating protein folding. They are the only suppliers of a higher eukaryotic expression system synthesising ensembles of molecular chaperones at levels capable of assisting the proper folding of abundantly expressed foreign proteins.

AB Vector's mission is to provide innovative tools to facilitate the production of correctly folded proteins.

Advantages
Up to now, developments in protein expression have been largely focused on improvements in generating recombinant clones and increasing the levels of protein production. AB Vector recently developed ProFold™, patent pending, the first commercially available vectors facilitating protein folding. The main advantage of ProFold™ vectors is the overexpression of molecular chaperones in either the cytoplasm or the ER, which help to fold the protein of interest. AB Vector have tested their vectors with mammalian, yeast and plant proteins and observed significant improvement in yield of biologically active proteins compared to the same proteins expressed using conventional baculovirus vectors. ProFold™ vectors enable large improvements in the solubility of proteins previously observed to be completely insoluble when expressed in E.coli.

Another advantage of ProFold™ vectors is a convenience of optimization of your protein expression system via green fluorescense. ProFold™ vectors provide for expression of Aequorea Victoria green fluorescent protein (GFP). This allows monitoring of virus propagation, optimization of cultivation conditions, and titrating virus stocks by "terminal green dilution". It saves time and effort; no need for expensive baculovirus titration kits or specialized equipment. The only essential pieces required are the same 265 nm UV transilluminator and digital camera routinely used for ethidium bromide stained gels. In addition, you can count green foci of infected cells using a fluorescence microscope or take advantage of flow cytometry.