Anaerobic fluorescent proteins
Evocatal supplies the evoglow®-series of fluorescent reporter proteins, the first type of fluorescent proteins that can be used in anaerobic as well as aerobic biological systems.
Fluorescent reporter proteins are valuable noninvasive molecular tools for in vivo real-time imaging of living cells and tissues as well as in vitro fluorescence labeling.
The evoglow® series make use of the novel flavin mononucleotide (FMN)-based fluorescent proteins (FbFPs) which were developed to overcome the major drawback of existing GFP-like reporter proteins. The latter have a strict requirement for molecular oxygen as a cofactor for the synthesis of their respective chromophores and hence cannot be used for application in anaerobic systems.
Evoglow has been applied successfully in the gram-negative bacteria such as Escherichia coli, Pseudomonas putida, Rhodobacter capsulatus; gram-positive bacteria such as Bacillus subtilis, Staphylococcus aureus; and eukaryotes such as Saccharomyces cerevisiae and Candida albicans.
You can review the publications reporting the use of evoglow proteins.
Products
Full information on the following evoglow kits:
- evoglow® basic kit
- evoglow® express kit
- evoglow® fusion kit
- evoglow® yeast kit
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