Resources

Watch short videos on techniques such as:

Fluorescent microscopy Cell culture & cell counting 2D gel electrophoresis
Antibody conjugation
ELISAs Primer design
Invasion & migration assays Flow cytometry Western blot and more …


Protocols are available for techniques including Western blot, ELISA, immunoprecipitation, FISH, and RNAi knockdown. Information is also available for antibody labelling, apoptosis, circulating tumour cells, epigenetics and more. 


Western Blot (WB) Tutorial Workshop via Skype
  • Are you new to Western Blotting? or
  • Do you need a refresher on Western Blotting?
You can now request a Western blot Tutorial with a technical specialist from Agrisera. Contact Sapphire Bioscience to register your interest & secure a booking.


General considerations for: Antibodies in Serum | Total IgG fraction | Total IgY fraction | Egg Yolk | Affinity Purified Antibodies | IgG | Chicken IgY

Successsful Antibody Production
Are you thinking about making your own antibodies? If so, view to poster to find out more about the three crucial components of Antibody Production.
  • Poster: Tips and Tricks for Antibody Production and Validation Process - How to obtain good results - View poster | Download poster

Technical Notes

Technical Tips for ELISA Assays​ [PDF]

Spike-and-recovery and linearity-of-dilution assays are two essential validation studies that assess the ability of the ELISA assay to measure the true (ie. accurate) amount of antigen in the sample.

​Bioss is dedicated to helping you achieve exceptional results. Their top-notch scientific support team has worked hard to develop these protocols for all their applications. They hope these instructional aids will assist you in your research!


IMMUNOHISTOCHEMISTRY

Bioss Immunohistochemistry - Frozen Sections (IHC-F) protocol

Bioss Immunohistochemistry - Parrafin Embedded Sections (IHC-P) protocol


IMMUNOFLUORESCENCE

Bioss Immunofluorescence protocol for Parrafin Sections


FLOW CYTOMETRY

Bioss Flow Cytometry Intracellular Phosphorylation Staining protocol

Bioss Flow Cytometry General Surface Staining protocol


WESTERN BLOT

Bioss Western Blot (WB) protocol


ELISA

Bioss Direct ELISA protocol

Bioss Indirect ELISA protocol​

SCIENTIFIC JOURNEYS
Brush up on your scientific history. Bioss presents a tribute to Scientific Excellence with their Scientific Journeys​.


Cayman Chemical offers various tools and resources to support their products and services and help make your research possible.


Technical Support

Analysis Tools: Analyze assay kit results with Microsoft Excel-based quantitation templates 

Assay Kit Recommendation Guide: Compare assay kits based on sample type, target, and preferred detection method

Batch Specific Data and Documents: Download or request product inserts, QC sheets, certificates of analysis, and GC-MS data

Chemical Structure Database: Download the latest compound database in MDL SD file format 

Literature & Media: Download or request catalogs, wall posters, application notes, scientific posters, and more


Forensic Chemistry

Cayman Spectral Library: Search the GC-MS spectral database available in Agilent MSD ChemStation and NIST file formats 

GC-MS Drug Identification Tool: Search unknowns against formula weight, base peak, or 2nd base ion

Synthetic Cannabinoid Flipbook​: Explore structural similarities to known Schedule 1 synthetic cannabinoids


    ​Browse through the Knowledge Base for general and product-specific FAQs, troubleshooting steps and protocols.

    Download literature including catalogues, product flyers, scientific posters and poster presentations.

    Analyse ELISA plates with your smart phoneDownload the App (Available for iOS 7+ and Android 4.2+)

    resource.jpg The Enzo ELISA Plate Reader is provided by Enzo Life Sciences as a tool to enable the use of colorimetric plate based assays in a wider variety of environmental settings. The ELISA Plate Reader mobile application is i​mage analysis based and is not intended to provide the sensitivity and reproducibility of a traditional plate reader. When used properly the plate reader application can provide quantitative results while providing portability and functionality when a lab based plate reader is inconvenient or unavail​able.​

    Antibody Search Tools
    Enzo Life Sciences offers thousands of antibodies against peptides, whole or partial proteins, and modified peptides or proteins. Backed by thousands of peer-reviewed citations, Enzo's Worry-free Antibody Trial Program​, and expert technical support, you can count on Enzo antibodies to deliver reliable, consistent, accurate data.
    • Rigorously validated for WB, IP, ELISA, IHC, ICC, IF, and flow cytometry
    • Superb performance documented in thousands of peer-reviewed publications from 1986-present
    • Expanded utility provided by common fluorophore and enzyme conjugates
    • Convenient secondary antibodies and positive control lysates and proteins
    • Bulk production and custom conjugation/formulation services to meet your needs


    Protocols to help you get the most out of your research

    Fabgennix_Protocols.jpg


    GeneTex has over 55,000 Antibodies for the following research areas:

    ​Cancer
    Immunology​
    ​Zebrafish
    Cell Biology
    Infectious Disease
    Stem Cell Development
    ​Disease Related
    Metabolism
    ​Epigenetics
    Neuroscience
    ​Epitope Tags
    Signal Transduction
     

    Check out GeneTex’s Mini Catalogues and Flyers (for e.g. VEGF, and more).

    Decorate your lab/office wall space with GeneTex’s informative signalling pathway posters or you can request hard copies.

    View the technical videos that include tips and troubleshooting for:

    • Immunohistochemistry (IHC). Also available, IHC (paraffin) troubleshooting print-out guide.
    • Immunofluorescence Assay (IFA)
    • Western Blot (WB) – Sample preparation, Electrophoresis

    A collection of useful printable protocols and technical tips available for applications: 

     

    ​Cell Culture
    Western Blot (WB)
    Fractionation of Membrane Cytoplasmic and Nuclear Proteins
    ​Cell Lysate Preparation
    Immunocytochemistry (ICC)
    Immunofluorescent Staining for Flow Cytometry
    Sandwich ELISA (ELISA)
    Immunohistochemistry (IHC)

     

    EasyBlot®, finally a solution to masking by denatured IgG, will help you get the cleanest results on your IP/Co-IP experiments.

    Sapphire Bioscience is the distributor of GeneTex in Australia and New Zealand and is there to support you 100% of the way.​

    ELISA development is a core specialty at Immunochemistry Technologies (ICT). They offer a full line of ELISA blocking buffers, wash buffers, and immunoassay components for immunoassay development and manufacturing. ICT has everything you need to DIY / Create-your-own ELISA from start to finish!

    Check out ICT's handy ELISA development technical resources:

    ​Draw chemical structures using the interactive drawing tool from Indofine Chemical Company

    ​Structure Searchbeta

    Jena Bioscience_structure_search_small.png
    Search all of Jena Bioscience's small molecule products (including all nucleosides and nucleotides) by sub-structure. Search now

    Jena Bioscience is the leading supplier for nucleotides and their analogues. They have more than 2.000 of natural and modified nucleotides available. 

    Nucleotides | Nucleotide Libraries & Kits | Nucleosides​​


    ​Opening Dangerous Goods Containers & Vials In 4 Steps​

    Read the Procedure​ 


    List Labs Blog

    • Pertussis Toxin Application in Multiple Sclerosis Research - Read the article​
    • Cell Ablation Using Diphtheria Toxin (DT) is an Important Technique for Studying Regeneration in Living Animals​ - Read the article​

    Check out the standardised IHC-plus™ Immunohistochemistry Protocol, used to validate all IHC-plus antibodies. LSBio Publications are available, including product references, white papers, press releases, target overviews, and product releases.

    Want to know more? Download the latest product booklets and flyers on the Media page.

    Mercodia Webinars - view the latest webinar recordings​

    Mercodia webinars connect you with experts in various scientific fields.  These special sessions are educational, informative and often provide you with answers to questions that may assist your group in making decisions now or in the future.  All of our webinars are recorded and posted below as an added resource for our valued customers.

    • Bariatric Surgery: Regulation of Gut and Islet Hormones by Dr. David A. D’Alessio 
    • Leptin and Diabetes by Dr. Jeffrey M. Friedman and Dr. Roger Unger
    • Beta Cell Regeneration: Novel Sources by Dr. Juan Domínguez-Bendala and Dr. Pedro Herrera
    • Advances in the Bionic Pancreas by Dr. Steven Russell
    • Glucagon Release with SGLT2 Inhibition by Dr. Ralph DeFronzo
    • Glucagon  - A Therapeutic Target -​ by Dr. Alan D Cherrington
    • Glucagon & Glicentin: Unique hormones from the Same Precursor - by Dr. Jens Juul Holst

    Mercodia Podcasts - listen to the latest webinars

    Exploring Frontiers in Translational Research

    Learn about scientific advances that lead to a better understanding of diseases and therapies. Interviews with scientists and clinicians will provide you with cutting-edge information, coming directly from the source.  Explore topics ranging from drug discovery and new FDA regulations to novel approaches to metabolic diseases (e.g., diabetes and heart disease).


    ProSci's antibody protocols are available for a wide variety of assays including western blot, ELISA, immunofluorescence, immunohistochemistry, and immunocytochemistry.​

    ​​

    Protocol  ​​ PDF Version​
    Western Blot Protocol      PDF Version
    Immunohistochemistry Protocol PDF Version
    Immunocytochemistry Protocol PDF Version
    Immunofluorescence Protocol (Tissue Sections) PDF Version
    Immunofluorescence Protocol (Fixed Cells) PDF Version
    ELISA Protocol    PDF Version

    Blog
    Learn more about ProSci's Antibody Resources

    ProSci offers a broad catalogue of over 25,000 research reagent products and offers extensive custom antibody services including the production of monoclonal, polyclonal and single domain antibodies, as well as additional services​.

    ​Have you watched Quansys' Q-Plex Multiplex Technology On-Demand Webinar yet

    In Introduction to Q-Plex Technology by Matt Groll, General Manager at Quansys Biosciences​


    White Papers

    Multiplex ELISA Validation - Learn all about selectivity, accuracy, precision, recovery & linearity, curve-fitting and stability. Also available as a printable pdf download.

    The Extracellular Matrix, Matrix Metalloproteinases and their roles in Disease​ - Learn about MMPs

    Chemokines as Biomarkers​​ - This whitepaper covers Eotaxin (CCL11), GRO-α (CXCL1), I-309 (CCL1), IL-8 (CXCL8), IP-10 (CXCL10), MCP-1 (CCL2), MCP-2 (CCL8), RANTES (CCL5) and TARC (CCL17). Also available as a printable PDF download​.


    Tech Tips for Pipetting

    Using a pipette the correct way can lead to better data and more reliable results. View the details​ | Download the PDF​​


    ​Molarity Calculator: Calculate the mass, volume or concentration required for a solution

    Dilution Calculator: Calculate the dilution required to prepare a stock solution

    Molecular Weight Calculator: Calculate the molar mass and elemental composition of a chemical using its chemical formula

    Technical Resources

    • Protocols - Slides on Direct Flow Cytometry, Indirect Flow Cytometry, ICC, IHC, WB, Direct ELISA, Indirect ELISA, Immunofluorescence
    • Video Protocol Series - Western Blotting