Resources

Watch short videos on techniques such as:

Fluorescent microscopy Cell culture & cell counting 2D gel electrophoresis
Antibody conjugation
ELISAs Primer design
Invasion & migration assays Flow cytometry Western blot and more …


Protocols are available for techniques including Western blot, ELISA, immunoprecipitation, FISH, and RNAi knockdown. Information is also available for antibody labelling, apoptosis, circulating tumour cells, epigenetics and more. 

Technical Resources


Western Blot (WB) Tutorial Workshop via Skype
  • Are you new to Western Blotting? or
  • Do you need a refresher on Western Blotting?
You can now request a Western blot Tutorial with a technical specialist from Agrisera. Contact Sapphire Bioscience to register your interest & secure a booking.

Antibody Storage Recommendations​
General considerations for: Antibodies in Serum | Total IgG fraction | Total IgY fraction | Egg Yolk | Affinity Purified Antibodies | IgG | Chicken IgY - View the Antibody Storage Recommendations

Successful Antibody Production
Are you thinking about making your own antibodies? If so, view to poster to find out more about the three crucial components of Antibody Production.
  • Poster: Tips and Tricks for Antibody Production and Validation Process - How to obtain good results - View poster | Download poster

Plant Science Events Calendar
Sign-up your meeting, workshop, symposium or check what is coming!
Plant Science Events Calendar.jpg

How to Cite an Antibody in a Scientific Publication?
As more than half of the antibodies used in research cannot be properly identified, it is absolutely crucial to include information, which will allow antibody identification by researchers who want to repeat your experiments. Some journals provide a guideline for antibody citation, unfortunately most of them do not. This issue is most often completely overlooked. Find out how to cite your antibody here.

Posters on Photosynthesis
Updated photosynthetic posters developed by Professor Govindjee, Professor Lars Olof Björn and  Dr Dmitry Shevela,are available in format A0 to decorate your office or lab wall. Contact Sapphire Bioscience to order a complimentary print copy of this poster.
Antibodies_Inc_camelids.jpg
Alpaca-derived nanobodies as part of the antibody ‘toolbox’

Rabbit and mouse antibodies are mainstay reagents found in all biological research labs, but some of you have likely heard the buzz around camelid antibodies. Camelids include camels, llamas, alpacas, and other lesser known species, though llamas and alpacas are the most commonly used camelids for antibody development since they are easier to handle....

Read the Antibodies Incorporated Blog >


BioLamina Technical ​Video Guides View All

  • Generating Biosilk 3D scaffolds for proliferation and differentiation of HPSCS | Watch Video
  • Benefits of the Biolaminin 521 cell culture system | Watch Video
  • Coating cultureware with Biolaminin substrates | Watch Video
  • Passaging and culture of human PSC on Biolaminin 521 | Watch Video
  • HPSC transition to Biolaminin 521 | Watch Video
  • Watch as cells grow - Culture ES and IPS cells on Laminin | Watch Video
Application Notes - View All

Technical Notes

Technical Tips for ELISA Assays​ [PDF]

Spike-and-recovery and linearity-of-dilution assays are two essential validation studies that assess the ability of the ELISA assay to measure the true (ie. accurate) amount of antigen in the sample.

​Bioss is dedicated to helping you achieve exceptional results. Their top-notch scientific support team has worked hard to develop these protocols for all their applications. They hope these instructional aids will assist you in your research!

PROTOCOLS

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Cayman Chemical offers various tools and resources to support their products and services and help make your research possible.


Technical Support

Analysis Tools: Analyse assay kit results with Microsoft Excel-based quantitation templates 

Assay Kit Recommendation Guide: Compare assay kits based on sample type, target, and preferred detection method

Batch Specific Data and Documents: Download or request product inserts, QC sheets, certificates of analysis, and GC-MS data

Chemical Structure Database: Download the latest compound database in MDL SD file format 

Literature & Media: Download or request lab wall posters, application notes, brochures, scientific posters, and more

Molarity Calculator: Determine the mass, concentration, or volume required to make a solution.

Molar Mass Calculator Tool: Determine the Molar mass and Percent mass composition of a linear chemical formula.

Fluorescence Spectra Viewer: Use this interactive spectra viewer to compare excitation and emission wavelengths of 900+ fluorophores. Multiplex different fluorophores and design experiments to help you find the best products for your application.

Cayman News: Read the newest announcements, articles, and press releases from Cayman


Forensic Chemistry & Toxicology

Cayman Spectral Library: Search the GC-MS spectral database available in Agilent MSD ChemStation and NIST file formats 

GC-MS Drug Identification Search Tool: Search unknowns against formula weight, base peak, or 2nd base ion

Synthetic Cannabinoid Flipbook​: Explore structural similarities to known Schedule 1 synthetic cannabinoids




Antibody Search Tools
Enzo Life Sciences offers thousands of antibodies against peptides, whole or partial proteins, and modified peptides or proteins. Backed by thousands of peer-reviewed citations, Enzo's Worry-free Antibody Trial Program​, and expert technical support, you can count on Enzo antibodies to deliver reliable, consistent, accurate data.
  • Rigorously validated for WB, IP, ELISA, IHC, ICC, IF, and flow cytometry
  • Superb performance documented in thousands of peer-reviewed publications from 1986-present
  • Expanded utility provided by common fluorophore and enzyme conjugates
  • Convenient secondary antibodies and positive control lysates and proteins
  • Bulk production and custom conjugation/formulation services to meet your needs


GeneTex has over 55,000 Antibodies for the following research areas:

​Cancer
Immunology​
​Zebrafish
Cell Biology
Infectious Diseases
Stem Cell Development
​Epigenetics
Neuroscience
​Epitope Tags
 


View the technical videos that include tips and troubleshooting for:

  • Immunohistochemistry (IHC)
  • Immunofluorescence Assay (IFA)
  • Western Blot (WB) – Sample preparation, Electrophoresis


A collection of useful printable protocols and technical tips available for antibody applications:

  • Protocol - Western Blot
  • Protocol - ICC/IF
  • Protocol - IHC (paraffin)
  • Protocol - IHC (frozen)
  • Protocol - Western Blot
  • Protocol - ICC/IF
  • Protocol - IHC (paraffin)
  • Protocol - IHC (frozen)
  • Protocol - IHC (whole-mount)
  • Protocol - Immunoprecipitation
  • Protocol - ELISA (sandwich)
  • Protocol - ELISA (indirect)
  • Protocol - Flow Cytometry
  • Protocol - Dot Blot
  • Protocol - Blocking Peptide Competition
  • Protocol - Cytosolic and Membrane Fractionation

Search for GeneTex products on the Sapphire Bioscience website >


​Opening Dangerous Goods Containers & Vials In 4 Steps​

Read the Procedure​ 


List Labs Blog

  • Pertussis Toxin Application in Multiple Sclerosis Research - Read the article​
  • Cell Ablation Using Diphtheria Toxin (DT) is an Important Technique for Studying Regeneration in Living Animals​ - Read the article​

Check out the standardised IHC-plus™ Immunohistochemistry Protocol, used to validate all IHC-plus antibodies. 

Mercodia Webinars - view the latest webinar recordings​

Mercodia webinars connect you with experts in various scientific fields.  These special sessions are educational, informative and often provide you with answers to questions that may assist your group in making decisions now or in the future.  All of our webinars are recorded and posted below as an added resource for our valued customers.

  • Bariatric Surgery: Regulation of Gut and Islet Hormones by Dr. David A. D’Alessio 
  • Leptin and Diabetes by Dr. Jeffrey M. Friedman and Dr. Roger Unger
  • Beta Cell Regeneration: Novel Sources by Dr. Juan Domínguez-Bendala and Dr. Pedro Herrera
  • Advances in the Bionic Pancreas by Dr. Steven Russell
  • Glucagon Release with SGLT2 Inhibition by Dr. Ralph DeFronzo
  • Glucagon  - A Therapeutic Target -​ by Dr. Alan D Cherrington
  • Glucagon & Glicentin: Unique hormones from the Same Precursor - by Dr. Jens Juul Holst


​NaveniFlex Kit Applications:

  • Detection of Interactions Between Glycoproteins in Ovarian Cancer Using Navinci Universal - Download the PDF

View available NaveniFlex kits here.

Blog

  • The Era of Platinum Genomes Has Arrived - Read more
  • Hi-C Technology Links Antimicrobial Resistance Genes to the Microbiome - Read more
  • Earth’s Wine Cellar: Digging into the Microbiome of Vineyards - Read more
  • HI-C SOLVES THE PROBLEM OF LINKING PLASMIDS TO HOSTS IN MICROBIOME SAMPLES - Read more
  • NEW VIDEO: FROM CONTIGS TO CHROMOSOMES - Read more
  • Uncovering the microbiome: What will you do with metagenomics? Read more

ProSci's antibody protocols are available for a wide variety of assays including western blot, ELISA, immunofluorescence, immunohistochemistry, and immunocytochemistry.​

​​

Protocol  ​​ PDF​
Western Blot Protocol      Download PDF
Immunohistochemistry Protocol Download PDF
Immunocytochemistry Protocol Download PDF
Immunofluorescence Protocol (Tissue Sections) Download PDF
Immunofluorescence Protocol (Fixed Cells) Dowload PDF
ELISA Protocol    Download PDF

Blog
Learn more about ProSci's Antibody Resources

ProSci offers a broad catalogue of over 25,000 research reagent products and offers extensive custom antibody services including the production of monoclonal, polyclonal and single domain antibodies, as well as additional services​.

Tech Tips for Multichannel Pipetting:
Using a pipette the correct way can lead to better data and more reliable results. | View Details


​Molarity Calculator: Calculate the mass, volume or concentration required for a solution

Dilution Calculator: Calculate the dilution required to prepare a stock solution

Molecular Weight Calculator: Calculate the molar mass and elemental composition of a chemical using its chemical formula